PLAT Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/10,000-1/100,000. Western blot: 1/1,000-1/10,000. Immunohistochemistry: 1/100-1/1,000 for Frozen, Parafolmaldehyde-Fixed Sections. Recommended pair for Sandwich Immunoassay: Capture: AM05122PU-N Detection: AP00892PU-N.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant human tPA
Specificity	This antibody recognizes tPA.
Formulation	0.05M Sodium Phosphate, pH 6.6 containing 0.1M Sodium Chloride and 1mM EDTA State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -70°C. Avoid repeated freezing and thawing.
Gene Name	plasminogen activator, tissue type
Database Link	Entrez Gene 5327 Human UniProt ID P00750
Background	TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, cell migration and many other physiopathological events. TPA binds to fibrin with high affinity; this leads to an increase in the catalytic efficiency of the enzyme of 100- to 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low density lipoprotein receptor related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. Binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.
Synonyms	tPA, Tissue-type plasminogen activator, Alteplase, Reteplase
Reference Data

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