CD40L (CD40LG) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human sCD40L by Direct ELISA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human sCD40L.  Sandwich ELISA: To detect Human sCD40L by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml is required. In conjunction with Purified Anti-Human sCD40L (Cat.-No AP01120PU-N or Cat.-No AP01120PU-S) as a Capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human sCD40L.  Western Blot: To detect Human sCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1-1.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD40L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived recombinant Human sCD40L Cat.-No PA151
Specificity	This antibody detects Human CD154 / sCD40L. Other species not tested.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	CD40 ligand
Database Link	Entrez Gene 959 Human UniProt ID P29965
Background	CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.
Synonyms	CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39
Reference Data

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