PLAT Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	ELISA. Immunohistochemstry on Frozen Sections: 1/50-1/100. Immunohistochemstry on Paraffin Sections: 1/50 (Pretreatment with 0.05% Pronase E for 20 minutes).
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Purified Human tissue Plasminogen Activator [tPA]
Specificity	This antibody detects free tissue Plasminogen Activator (tPA) and tPA inhibitor complexes. There were no cross reactivities obtained with Human uPA and PAI-2.
Formulation	State: Serum State: Lyophilized Serum
Reconstitution Method	Restore in aqua bidest to initial volume.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	plasminogen activator, tissue type
Database Link	Entrez Gene 5327 Human UniProt ID P00750
Background	TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. TPA binds to fibrin with high affinity; this leads to an increase in the catalytic efficiency of the enzyme of 100- to 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low density lipoprotein receptor related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. Binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold
Synonyms	tPA, Tissue-type plasminogen activator, Alteplase, Reteplase
Note	Reference no.: LocusID 5327
Reference Data

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