MEK1 (MAP2K1) pSer222 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-Sp-F-V).
Specificity	MEK1 (phospho-Ser221) antibody detects endogenous levels of MEK1 only when phosphorylated at serine 221.
Formulation	PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase kinase 1
Database Link	Entrez Gene 5604 Human UniProt ID Q02750
Background	MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. MEK1 is activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
Synonyms	MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1
Reference Data

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