MEK1 (MAP2K1) pSer218/222 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around the phosphorylation site of Serine 217/221 (I-D-Sp-M-A) derived from Human MEK1/MEK2
Specificity	This antibody detects endogenous levels of MEK1/MEK2 only when phosphorylated at Serine 217/221.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl and 50% Glycerol State: Aff - Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	45 kDa
Gene Name	mitogen-activated protein kinase kinase 1
Database Link	Entrez Gene 5604 Human UniProt ID Q02750
Synonyms	MAPKK1, ERK activator kinase 1, ERK activator kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MEK1, MEK2, PRKMK1, PRKMK2, MAP kinase kinase 1, MAP kinase kinase 2
Reference Data

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