Dnm1 pSer774 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence. Immunohistochemistry on Frozen Sections. Western Blot (1/1000): This antibody detects a band of approximately 95kDa in Rat hippocampus lysates. For the detection of Phosphoproteins, Serine Phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a Phosphoprotein and its use can result in high background.
Reactivity	Bovine, Canine, Chicken, Human, Monkey, Mouse, Rat
Host	Sheep
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide corresponding to an amino acid sequence within Dynamin which includes phosphorylated Ser774.
Specificity	This antibody is specific for Dynamin when phosphorylated at Serine 774.
Formulation	10mM HEPES, pH7.5 containing 0.09% Sodium Azide, 0.01% BSA and 50% Glycerol. State: Purified State: Liquid purified IgG fraction.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	dynamin 1
Database Link	Entrez Gene 140694 Rat UniProt ID P21575
Background	Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.
Synonyms	DNM1, DNM
Reference Data

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