CYP27A1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000.  Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to amino acids 100-150 of Human CYP27A1.
Specificity	This antibody detects endogenous levels of CYP27A1 protein. (region surrounding Glu131)
Formulation	Phosphate buffered saline (PBS), pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~60 kDa
Gene Name	cytochrome P450 family 27 subfamily A member 1
Database Link	Entrez Gene 1593 Human UniProt ID Q02318
Background	The immunosuppressant cyclosporin A (CsA) forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity.Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of the CsA and is encoded by a single unique gene conserved from yeast to humans. CyPA is involved in T cell differentiation and proliferation and is highly expressed in brain. CyPA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.
Synonyms	CYP27, Cytochrome P450 27, Cytochrome P-450C27/25, Sterol 26-hydroxylase, Sterol 27-hydroxylase, Vitamin D3-25-hydroxylase
Reference Data
Protein Families	Druggable Genome, P450
Protein Pathways	Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis

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