EGR1 (94-108) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (1:4000 - 1:16000) IHC IHC-P (10 µg/ml) WB (1:500 - 1:3000)
Reactivity	Mouse, Human, Chimpanzee
Host	Rabbit
Clonality	Polyclonal
Immunogen	This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1.
Specificity	A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.
Formulation	0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Gene Name	early growth response 1
Database Link	Entrez Gene 1958 Human UniProt ID P18146
Background	EGR1 antibody LS-B28 is an unconjugated rabbit polyclonal antibody to EGR1 (ZIF268) from human. It is reactive with human, mouse and chimpanzee. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Synonyms	EGR1, AT225, Early growth response 1, G0S30, KROX24, NGFIA, KROX-24, TIS8, ZIF-268, ZIF268, Zinc finger protein Krox-24, EGR-1, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225
Note	Immunohistochemistry: LS-B28 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B28 was determined to be 10 ug/ml.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.