Horseradish Peroxidase / HRP Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: 1/10000-1/40000.  Dot Blot.  Western Blot: 1/1000-1/4000. Immunoprecipitation.
Host	Sheep
Clonality	Polyclonal
Immunogen	Native Peroxidase [Horseradish]
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish].  Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Formulation	0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4 State: Azide Free State: Lyophilized antiserum prepared from monospecific antiserum by a Delipidation and Defibrination. Stabilizer: None Preservative: None
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Reference Data

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