Myb Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/10,000 – 1/25,000. Western Blot: 1/250 – 1/1000.
Reactivity	Chimpanzee, Equine, Human, Monkey, Mouse, Opossum, Orang-Utan, Porcine, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide corresponding to an internal portion of the c-Myb protein
Specificity	This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide State: Aff - Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Gene Name	myeloblastosis oncogene
Database Link	Entrez Gene 17863 Mouse UniProt ID P06876
Background	The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Posttranslational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb.
Synonyms	c-Myb
Reference Data

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