SAE1 pSer185 Rabbit Polyclonal Antibody

CAT#: AP09416PU-N

SAE1 pSer185 rabbit polyclonal antibody, Aff - Purified

Size: 100 ug 25 ul



Need it in bulk or conjugated?
Get a free quote

CNY 8,025.00


货期*
4周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution ELISA: 1/100,000 - 1/125,000.
Western Blot: 1/750 - 1/1,000.
Reactivity Bovine, Canine, Chimpanzee, Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein
Specificity This antibody is directed against SUMO Activating Enzyme E1 protein.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
State: Aff - Purified
State: Liquid purified Ig
Concentration lot specific
Purification Affinity chromatography
Conjugation Unconjugated
Storage Condition Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Storage Conditions for Trial Size:
This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Gene Name SUMO1 activating enzyme subunit 1
Background SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.
Synonyms SUA1, UBLE1A
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...