Biotin (F(ab')2 fragment) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, WB
Recommend Dilution	Immunofluorescence. Flow Cytometry: 1/100 - 1/250. Western Blot: 1/100-1/250. ELISA: 1/100-1/250.
Host	Goat
Clonality	Polyclonal
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity	This is a Phycoerythrin Conjugated F(ab)2 fragment of Anti-Biotin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide Label: PE State: Liquid F(ab)2 fragment Label: R-Phycoerythrin ( Molecular Weight 240,000 daltons) Absorption emission: 488 nm / 575 nm
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Store reagent in the dark.
Background	Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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