Calreticulin (CALR) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IHC, WB
Recommend Dilution	Western blot: 1/1000.  Flow Cytometry: 1/10-1/50. Immunohistochemistry on Paraffin Sections: 1/50-1/100. ELISA: 1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	This CALR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the Central region of Human CALR.
Specificity	This antibody detects Calreticulin at Center.
Formulation	PBS with 0.09% (W/V) Sodium Azide State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Purified through a protein A column, followed by peptide affinity purification
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	calreticulin
Database Link	Entrez Gene 12317 MouseEntrez Gene 64202 RatEntrez Gene 811 Human UniProt ID P27797
Background	Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
Synonyms	CRP55, Calregulin, HACBP, ERp60, grp60, CALR, CRTC
Note	Molecular weight: 48142 Da
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transcription Factors
Protein Pathways	Antigen processing and presentation

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