14-3-3 theta (YWHAQ) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000.  Immunofluorescence: 1/50-1/200.   Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 200-240 of Human 14-3-3 θ.
Specificity	This antibody detects endogenous levels of 14-3-3 θ/τ protein.
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~28 kDa
Gene Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
Database Link	Entrez Gene 22630 MouseEntrez Gene 25577 Rat UniProt ID P27348
Background	It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple cell signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.
Synonyms	14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection

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