ETFB (152-165) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/8000 (Detetion Limit).   Western blot: 1-3 µg/ml. This antibody detects a band of ~27kDa in Human, Mouse and Rat Liver lysates. Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region (aa 152-165) of the protein sequence according to NP_001976.1 and NP_001014763.1.
Specificity	This antibody is expected to recognize both reported isoforms (NP_001976.1 and NP_001014763.1).
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	electron transfer flavoprotein beta subunit
Database Link	Entrez Gene 110826 MouseEntrez Gene 292845 RatEntrez Gene 2109 Human UniProt ID P38117
Background	ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Synonyms	Beta-ETF
Note	Calculated Molecular Weight: 27.8kDa (NP_001976.1).
Reference Data
Protein Families	Druggable Genome

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