3-beta-HSD Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Working Dilutions: Non-precipitating antibody-binding techniques: at least 1/400.
Reactivity	P. testosteroni
Host	Rabbit
Clonality	Polyclonal
Immunogen	beta-Hydroxysteroid Dehydrogenase isolated and purified from Pseudomonas testosteroni cells. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	beta-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni cells. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized hyperimmune IgG fraction Molar radio: Biotin/IgG ~5.2
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated freezing and thawing.
Database Link	UniProt ID P19871
Synonyms	3-beta-hydroxysteroid dehydrogenase
Reference Data

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