LDHB Rabbit Polyclonal Antibody

CAT#: AP21329BT-N

LDHB rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



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CNY 5,486.00


货期*
5周

规格
    • 1 ml

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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommend Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
Working Dilutions:
Non-precipitating antibody-binding techniques: 1/50-1/500.
Reactivity Bovine
Host Rabbit
Clonality Polyclonal
Immunogen L-Lactic Dehydrogenase is isolated and purified from Bovine heart.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity L-Lactic Dehydrogenase from Bovine heart.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: Biotin
State: Lyophilized hyperimmune IgG fraction
Molar radio: Biotin/IgG ~5.6
Reconstitution Method The antibody is reconstituted by adding 1.0 ml sterile distilled water and spun down to remove insoluble particles. The antibody should be divided into small aliquots for freezing.
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Biotin
Storage Condition Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Background Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
Synonyms LDHB, L-lactate dehydrogenase B chain, LDH-B, EC=1.1.1.27, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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