PRSS1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or histochemical techniques). Recommended Working Dilutions: vary widely, but may be between 1/1000 and 1/5000.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Trypsin isolated and purified from Bovine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Biotin conjugated IgG fraction of Polyclonal Rabbit antiserum to Trypsin from Bovine pancreas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized Hyperimmune purified IgG fraction Label: Marker: N-Hydroxysuccinimidobiotin. Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life Molar radio: Biotin/ IgG ~4.4
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 780933 BovineEntrez Gene 615026 Bovine UniProt ID P00760
Synonyms	TRP1, TRY1, TRYP1, Trypsin I, Cationic trypsinogen, Beta-trypsin, Serine protease 1
Reference Data

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