VCAM1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human VCAM-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant Human VCAM-1. Sandwich ELISA: To detect Human VCAM-1 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This antigen Biotin purified antibody, in conjunction with Affinity Anti-Human VCAM-1 (cat.-No AP22317PU-N or AP22317PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of ecombinant Human VCAM-1. Western Blot: To detect Human VCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human VCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure HEK293 cells derived Recombinant Human CD106 / VCAM1
Specificity	Recognizes Human CD106 / VCAM1.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (sterile filtered) purified Ig fractionin
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vascular cell adhesion molecule 1
Database Link	Entrez Gene 7412 Human UniProt ID P19320
Background	CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110) is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. It is also present on follicular and interfollicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin alphaBeta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in tumor metastasis.
Synonyms	V-CAM 1, INCAM-100, L1CAM, VCAM-1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Leukocyte transendothelial migration

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