NLRP3 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC
Recommend Dilution	Peptide ELISA: Detection Limit: 1/128000. Western blot:  This antibody is not recommended for use in Western blotting. Flow Cytometry analysis of U937 cells (10 µg/ml).  Immunofluorescence: 10µg/ml. Strong expression of the protein seen in the cytoplasm of U937 cells.  Immunohistochemistry on Paraffin Sections: 2-5 µg/ml.
Reactivity	Bovine, Human, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	synthetic peptide corresponding to the C-Terminus of the protein sequence according to NP_001073289.1; NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1.
Specificity	Recognizes CIAS1/Cryopyrin (C-Term).
Formulation	Tris saline, pH 7.3 State: Aff - Purified State: Liquid purified IgG fraction. Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	NLR family, pyrin domain containing 3
Database Link	Entrez Gene 287362 RatEntrez Gene 114548 Human UniProt ID Q96P20
Synonyms	NLRP3, C1orf7, CIAS1, PYPAF1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	NOD-like receptor signaling pathway

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