SCD1 (SCD) (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IHC
Recommend Dilution	Peptide ELISA: Limit Dlution: 1/64000. Western Blot: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 µg/ml antibody staining. Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. Staining of paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes.  Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-RIKRTGDGNYKSG, from the C Terminus of the protein sequence according to NP_005054.3.
Specificity	Recognizes Stearoyl-CoA desaturase.
Formulation	Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	stearoyl-CoA desaturase
Database Link	Entrez Gene 6319 Human UniProt ID O00767
Background	Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5 (MIM 608370), have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Zhang et al. (1999) [PubMed 10229681]; Wang et al., 2005).
Synonyms	Acyl-CoA desaturase, Fatty acid desaturase, Stearoyl-CoA desaturase
Reference Data
Protein Families	Transmembrane
Protein Pathways	Biosynthesis of unsaturated fatty acids, PPAR signaling pathway

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