Glutathione Peroxidase 4 (GPX4) (184-196) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	IHC IHC-P (2.5 µg/ml) Peptide-ELISA (1:16000) WB (0.1 - 0.3 µg/ml)
Reactivity	Human, Monkey
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-EEPLVIEKDLPHY, from the C-Terminus of protein sequence according to NP_002076.2NP_001034937.1.
Specificity	Human GPX4. This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
Formulation	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene Name	glutathione peroxidase 4
Database Link	Entrez Gene 2879 Human UniProt ID P36969
Background	MCSP antibody LS-B3246 is an unconjugated goat polyclonal antibody to MCSP (GPX4) (aa184-196) from human. It is reactive with human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Synonyms	GPX4, GPx-4, MCSP, SnPHGPx, Phospholipid hydroperoxidase, SnGPx, Glutathione peroxidase 4, GSHPx-4, PHGPx
Note	Immunohistochemistry: LS-B3246 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3246 was determined to be 2.5 ug/ml.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Arachidonic acid metabolism, Glutathione metabolism

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