HSP27 (HSPB1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: At 1μg/ml with the appropriate system to detect HSP27 in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect HSP27 in formalin fixed and paraffin embedded tissues.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence at the C-terminal of human HSP27
Specificity	This antibody detects HSPB1 / HSP27 (C-term).
Formulation	50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	heat shock protein family B (small) member 1
Database Link	Entrez Gene 15507 MouseEntrez Gene 24471 RatEntrez Gene 3315 Human UniProt ID P04792
Background	The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. The deduced 199-amino acid HSP27 protein shows sequence similarity to mammalian alpha-crystallins. Approximately 20% of its residues are susceptible to phosphorylation. The HSP27 gene, which is mapped to 7q11.23 and has 3 exons1, produced a 2.2-kb transcript in an in vitro transcription assay. Decreasing ROS in cells expressing mutant huntingtin, HSP27 protects cells against oxidative stress. In other words, HSP27 is a suppressor of polyglutamine (polyQ)-mediated cell death. Furthermore, MAPKAPK5 is a major stress-activated kinase that can phosphorylate HSP27 in vitro.
Synonyms	Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25
Reference Data
Protein Pathways	MAPK signaling pathway, VEGF signaling pathway

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