Orexin Receptor 1 (HCRTR1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: At 2μg/ml with the appropriate system to detect Orexin Receptor in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect Orexin Receptor in formalin fixed and paraffin embedded tissues.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence at the C-terminal of human Orexin Receptor
Specificity	This antibody detects Orexin Receptor 1+2 (C-term). No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	hypocretin receptor 1
Database Link	Entrez Gene 25593 RatEntrez Gene 230777 MouseEntrez Gene 3061 Human UniProt ID O43613
Background	Orexins are endogeneous peptides that activate Orexin Receptor type 1 and type 2, two closely related G-protein coupled receptors. Orexins-A and -B are hypothalamic peptides derived from a precursor called preproorexin and affect the stimulation of food intake. They act on G protein receptors named orexin receptor-1 (OX1R) and orexin receptor-2 (OX2R), respectively.Stimulation of these receptors by orexins causes an increase in intracellular calcium levels in hypothalamic cells in vitro. In vivo, orexins control feeding and may also be involved in modulating sleep-wake cycles and other hypothalamic functions.
Synonyms	HCRTR1, HCRTR2, Ox1r, Ox2r
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction

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