EGFR Rabbit Polyclonal Antibody

CAT#: AP23433PU-S

EGFR rabbit polyclonal antibody, Aff - Purified

Size: 50 ug 100 ug

Conjugation: Unconjugated Biotin



Need it in bulk or conjugated?
Get a free quote

CNY 3,910.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Sandwich ELISA: To detect Human EGFR by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGFR (AP23433BT-N or AP23433BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human EGFR.
Western Blot: To detect Human EGFR by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human EGFR is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure recombinant Human EGFR.
Specificity Recognizes EGF Receptor.
Formulation PBS, pH 7.2 without preservatives
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing an immobilized Human EGFR matrix.
Conjugation Unconjugated
Storage Condition Store the lyophilized antibody at -20°C.
Following reconstitution it is stable for two weeks at 2-8°C.
Frozen aliquots are stable for 6 months when stored at -20°C.
Avoid repeated freezing and thawing.
Gene Name epidermal growth factor receptor
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...