Orexin Receptor 1 (HCRTR1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: Detection Limit: 1/8000. Western Blot: 0.1-0.3 µg/ml. Approx 48+35kDa band observed in Human Brain lysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Silveyra et al, Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E977-85; PMID: 17638707).
Reactivity	Canine, Human, Mouse, Rat, Sheep
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_001516.1.
Specificity	This antibody detects Orexin receptor 1.
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	hypocretin receptor 1
Database Link	Entrez Gene 3061 Human UniProt ID O43613
Background	Orexins are endogeneous peptides that activate Orexin Receptor type 1 and type 2, two closely related G-protein coupled receptors. Orexins-A and -B are hypothalamic peptides derived from a precursor called preproorexin and affect the stimulation of food intake. They act on G protein receptors named orexin receptor-1 (OX1R) and orexin receptor-2 (OX2R), respectively.timulation of these receptors by orexins causes an increase in intracellular calcium levels in hypothalamic cells in vitro. In vivo, orexins control feeding and may also be involved in modulating sleep-wake cycles and other hypothalamic functions.
Synonyms	Hypocretin receptor type 1, HCRTR1, Ox1r
Note	Predicted Molecular Weight: 47.5kDa (NP_001516.1)
Reference Data

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