Adenosine Receptor A2a (ADORA2A) (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	Peptide ELISA: 1/8000 (Detection Limit).  Western Blot: This antibody has been successfully used on lysates of primary Rhesus microglia (See Reference 1 for more details).  Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of HepG2 cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C-Terminus of the protein sequence according to NP_000666.2.
Specificity	This antibody detects Adenosine A2a Receptor.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	adenosine A2a receptor
Database Link	Entrez Gene 135 Human UniProt ID P29274
Background	The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
Synonyms	ADORA2A, ADORA2
Reference Data

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