DR5 (TNFRSF10B) Rabbit Polyclonal Antibody

CAT#: AP31096BT-N

DR5 (TNFRSF10B) rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Direct ELISA: To detect Human sTRAIL Receptor-2 by Direct ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sTRAIL Receptor-2.
Sandwich ELISA: To detect Human sTRAIL Receptor-2 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Human sTRAIL Receptor-2 (AP31096BT-N or AP31096BT-S) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sTRAIL Receptor-2.
Western Blot: To detect Human sTRAIL Receptor-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL Receptor-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Canine, Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) 14,9 kDa recombinant Human soluble TRAIL Receptor-2.
Specificity Recognizes sTRAIL Receptor-2.
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing an immobilized Human sTRAIL Receptor-2 matrix
Conjugation Biotin
Storage Condition Store the lyophilized antibody at -20°C.
Following reconstitution it is stable for two weeks at 2-8°C.
Frozen aliquots are stable for 6 months when stored at -20°C.
Avoid repeated freezing and thawing.
Gene Name tumor necrosis factor receptor superfamily member 10b
Background Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.
Synonyms TNFRSF10B, DR5, KILLER, TRICK2, ZTNFR9, Death receptor 5, TRAIL receptor 2, TRAIL-R2
Reference Data
Protein Families Druggable Genome, Transmembrane
Protein Pathways Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity, p53 signaling pathway
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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