IDH1 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.3-1 µg/ml. A band of ~38kDa is observed in wildtype lysates of S. cerevisiae (Data kindly provided by F. Reggiiori, University Medical Centre Utrecht, Netherlands).
Reactivity	Yeast
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the Internal region of the protein sequence according to NP_014361.1.
Specificity	Recognizes IDH1 (Yeast).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P28834
Background	Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH1 contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Synonyms	Isocitric dehydrogenase, YNL037C
Note	Calculated Molecular Weight: 39.3kDa (NP_014361.1).
Reference Data

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