RNF36 (TRIM69) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: 0.5-2 µg/ml. Approx 55kDa band observed in Mouse Testis lysates. Immunohistochemistry on Paraffin Sections: 2.5-3.8 µg/ml. In paraffin embedded Human Testis shows speckled staining in nuclei of spermatocytes and spermitids.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C Terminus of the protein sequence according to NP_892030.3; NP_542783.2.
Specificity	This antibody is expected to recognize both reported isoforms (NP_892030.3, NP_542783.2).
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tripartite motif containing 69
Database Link	Entrez Gene 70928 MouseEntrez Gene 311373 RatEntrez Gene 140691 Human UniProt ID Q86WT6
Background	TRIM69 is a member of the RING-B-box-coiled-coil (RBCC) family with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of TRIM69 is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis.
Synonyms	RING finger protein 36
Note	Calculated Molecular Weight: 57.3kDa (Mouse NP_536771.2).
Reference Data
Protein Families	Druggable Genome

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