OVAL Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/25,000. Western blot: 1/500-1/2,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/300-1/2,000.
Reactivity	Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Ovalbumin from Hen Egg White (native protein)
Specificity	Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity gainst Ovalbumin from other sources is unknown.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 State: Purified State: Liquid (sterile filtered) purified IgG fraction Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration	lot specific
Purification	Multi-Step Process which includes Delipidation, Salt Fractionation and Ion Exchange Chromatography followed by extensive dialysis against the buffer
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.  Centrifuge product if not completely clear after standing at room temperature. Avoid repeated freezing and thawing.
Gene Name	ovalbumin (SERPINB14)
Database Link	Entrez Gene 396058 Chicken UniProt ID P01012
Background	Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation.  Anti Ovalbumin Antibody recognizes Ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.
Synonyms	SERPINB14, Egg albumin, Plakalbumin, Allergen Gal d II
Reference Data

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