MAPK6 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.  Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 µg MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N. Western blots of the IP were labelled with Rabbit anti-ERK3 serum (CST) or with Mouse anti-MK5 (SC). An approx 100kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in  in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunohistochemistry on Paraffin Sections: 10 µg/ml in  Paraffin embedded Human Heart.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.
Specificity	Recognizes MAPK6 / ERK3.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Gene Name	mitogen-activated protein kinase 6
Database Link	Entrez Gene 5597 Human UniProt ID Q16659
Synonyms	Mitogen-activated protein kinase 6, MAP kinase isoform p97, p97-MAPK
Reference Data

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