PRAK (MAPKAPK5) (192-206) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Peptide ELISA: 1/64000 (Detection Limit). Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after AP32809PU-N labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 µg AP32808PU-N or AP32809PU-N. Western blots of the Immunoprecipitation were labelled with Mouse anti-MK5 (SC) or with Rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunohistochemistry on Paraffin Sections: 4-8 µg/ml. In paraffin embedded Human Kidney shows nuclear staining in the DCT.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region (192-206) of the protein sequence according to NP_003659.2; NP_620777.1.
Specificity	Recognizes MK5 / MAPKAPK5 (192-206).
Formulation	Tris saline, pH~7.3, 0 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase-activated protein kinase 5
Database Link	Entrez Gene 8550 Human UniProt ID Q8IW41
Synonyms	MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK
Reference Data

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