GFP Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA. Immunofluorescence: 1/200-1/2000. Western Blot: 1/1000-1/10000. Immunohistochemistry on frozen tissue: 1/200.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant GFP
Specificity	This antibody reacts with GFP and GFP fusion proteins.
Formulation	State: Serum State: Lyophilized serum Preservative: None
Reconstitution Method	Restore in distilled water to original volume.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P42212
Background	GFP is responsible for bioluminescence in the jellyfish Aequorea victoria. This protein has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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