EPCAM Mouse Monoclonal Antibody [Clone ID: HEA125]

Specifications

Product Data
Clone Name	HEA125
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Cytological Material. Immunofluorescence microscopy. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue. Cellsorting Pretreatment: With paraffin embedded sections, protease pre-treatment is required prior to antibody application. Working Dilutions: Ready-to-use for immunohistochemical application. Incubation Time: 1 h at RT, extended with paraffin.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HT-29 colon carcinoma cell line.
Specificity	Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein). HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide Reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Background	Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data
Protein Families	ES Cell Differentiation/IPS, Transmembrane

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