HCV Envelope glycoprotein E1 Mouse Monoclonal Antibody [Clone ID: BDI198]

Specifications

Product Data
Clone Name	BDI198
Applications	ELISA, IF, IHC, WB
Recommend Dilution	IFA. ELISA. Western blot. Immunohistochemistry.
Reactivity	Hepatitis C Virus
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant HCV E1 glycoprotein (genotype 1b).
Specificity	Specific to HCV E1.
Formulation	0.01M PBS, pH 7.2 containing 0.09% Sodium Azide as preservative without stabilizing proteins State: Purified State: Liquid purified Ig fraction from Ascites (> 90% pure)
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Envelope glycoproteins E1 and E2 are involved in virus attachment to the host cell as well as in virus endocytosis and fusion with host membrane. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
Synonyms	Heptitis C Virus E1, gp32, gp35
Reference Data

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