Sftpd (Lectin Domain) Mouse Monoclonal Antibody [Clone ID: VIF11]

Specifications

Product Data
Clone Name	VIF11
Applications	ELISA, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1 µg/ml (1/400) for rat cryosections. Immunohistochemistry on Paraffin Sections: Not tested. ELISA. Western Blots.  Suggested Positive Control: Rat lung.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified rat surfactant protein D from bronchoalveolar lavage.
Specificity	Antigen Distribution on Tissue Sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.
Formulation	PBS, pH 7.2, 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative Label: Biotin State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	surfactant protein D
Database Link	Entrez Gene 6441 HumanEntrez Gene 25350 Rat UniProt ID P35248
Background	Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43kDa in its reduced state, 620kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.
Synonyms	Lung surfactant protein D, SP-D, PSP-D, PSPD, SFTP4, Collectin-7, COLEC7
Reference Data

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