Iba1 (AIF1) Mouse Monoclonal Antibody [Clone ID: 1022-5]

CAT#: BM4114

Iba1 (AIF1) mouse monoclonal antibody, clone 1022-5, Aff - Purified

Conjugation: Unconjugated Biotin FITC



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CNY 11,025.00


货期*
5周

规格
    • 200 ug

Product images

Specifications

Product Data
Clone Name 1022-5
Applications IHC
Recommend Dilution Immunohistochemistry on Paraffin Sctions: 10-20 μg/ml (1/20-1/0). Mcrowave pretreatment for antigen retrieval recommended.
Suggested Positive Control: Human spleen.
Does not react on routinely processed Frozen Sections.
Reactivity Human, Porcine, Rat
Host Mouse
Clonality Monoclonal
Immunogen Purified Human recombinant AIF-1.
Epitope not further characterized.
Specificity The antibody reacts with AIF-1.
Formulation PBS, pH 7.2 containing 5 mg/ml BSA as stabilizer and 0.05% (v/v) Kathon as preservative
State: Aff - Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore by adding 0.5 ml distilled water.
Concentration 0.4 mg/ml (after reconstitution)
Purification Affinity Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Gene Name allograft inflammatory factor 1
Background Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activated macrophages and microglial cells. It has been suggested that AIF-1 expression is associated with chronic inflammatory processes. AIF-1, like the Ca2+-binding peptides MRP8 and MRP14, is expressed by activated macrophages and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts. The recently described apoptosis-inducing factor AIF is not related to AIF-1.
Synonyms AIF-1, Protein G1, IBA1, Allograft inflammatory factor 1
Note Protocol: Immunohistochemitry With Unlabeled Monoclonal Antibodies On Paraffin-Embedded Tissue Sections
1. Deparaffinize slides in chloroform for 30min and rehydrate to distilled water
2. Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0.01M citrate buffer pH 6.0
3. Incubate slides for 15min in 200ml methanol/4.8ml 30%H2O2
4. Rinse slides 2x in distilled water and for 5min in TBS, pH 7.5
5. Block with normal swine serum diluted 1:10 in Tris buffered saline pH 7.5, containing 0.1% BSA. Cover sections with NSS and incubate for 15min in a humid chamber at RT 
6. Remove serum from sections (do not rinse)
7. Incubate with primary antibody diluted in TBS/0.1%BSA, pH 7.5 for 60min in a humid chamber at RT 
8. Rinse sections in TBS, pH 7.5 for 5min, 2x
9. Incubate with secondary antibody (Rabbit anti Mouse IgG+IgM-Biotin) 1:400 diluted in TBS/0.1%BSA, pH 7.5 for 30min in a moist chamber, RT. Preincubate now StreptABComplex /HRPO in TBS/0.1%BSA, pH 7.5!
10. Rinse sections in TBS, pH 7.5 for 5min, 2x
11. Incubate with StreptABComplex /HRPO for 30min in a humid chamber at RT  
12. Rinse sections in TBS, pH 7.5 for 5min, 2x
13. DAB-staining Prepare 3,3' - diaminobenzidintetrahydrochloride (DAB) according the manufacturers instructions (e.g. DAB tablets) and incubate slides for 1-15 min. Check color reaction to avoid overstaining.
14. Rinse sections in TBS, pH 7.5 for 5min, 2x
15. Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min
16. Mount slides with coverslips
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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