Complement C3 (C3) Mouse Monoclonal Antibody [Clone ID: H206]

Specifications

Product Data
Clone Name	H206
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot. Immunohistochemistry on Frozen Sections: (also after Fixation with Paraformaldehyde). Working Dilution: 1/10 in PBS, pH 7.4. Incubation Time: 1h at RT.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human complement component C3
Specificity	H206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kD fragment of C3c). Disorders Specifically Detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with Positive Control anti C3b-beta, (H11; Cat. No. BM2132) and Negative Control anti-C3a (H13; Cat. No. BM2131). Activated, deposited C3b bears C3b-alpha and C3b-beta, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H 13.
Formulation	Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore in 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	complement component 3
Database Link	Entrez Gene 718 Human UniProt ID P01024
Background	The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.
Synonyms	CPAMD1, Complement component 3
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.