ACTC1 Mouse Monoclonal Antibody [Clone ID: AC1-20.4.2]

Specifications

Product Data
Clone Name	AC1-20.4.2
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections: 1/10. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers for application on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhance specifity. This is not necessary for Paraffin sections.
Reactivity	Bovine, Chicken, Human, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.
Specificity	This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms. Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).
Formulation	Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in 1.0 ml distilled water.
Purification	Affinity Chromatographyon Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody After reconstitution at 2-8°C.
Gene Name	actin, alpha, cardiac muscle 1
Database Link	Entrez Gene 70 Human UniProt ID P68032
Background	Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.
Synonyms	ACTC; ASD5; CMD1R; CMH11
Reference Data

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