Synaptopodin (SYNPO) Mouse Monoclonal Antibody [Clone ID: G1D4]

Specifications

Product Data
Clone Name	G1D4
Applications	IF, IHC, WB
Recommend Dilution	Immunofluorescence.  Immunohistochemistry on Frozen Sections.  Immunohistochemistry on Paraffin Sections (After microwave treatment).  Working Dilutions: 1/5-1/10 for 1h at RT.  Western blot: 0.1-0.5 µg/ml.  The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
Reactivity	Bovine, Guinea Pig, Gerbil, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Isolated Rat kidney glomeruli.
Specificity	The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells.  Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons. Negative Species: Rabbit, Frog and Chicken.
Formulation	Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month  or (in aliquots) at -20°C for longer.  Avoid repeated freezing and thawing.
Gene Name	synaptopodin
Database Link	Entrez Gene 11346 Human UniProt ID Q8N3V7
Background	Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Synonyms	KIAA1029
Reference Data

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