Cytokeratin 2 (KRT2) (N-term) Mouse Monoclonal Antibody [Clone ID: Ks 2.342.7.4]

Specifications

Product Data
Clone Name	Ks 2.342.7.4
Applications	IF, IHC, WB
Recommend Dilution	Western Blot (Alkaline Phosphatase): 1/200 Western Blot (ECL): 1/60.000 Immunofluorescence: 1/200 Immunohistochemistry on Frozen Sections: 1/100. Pretreatment with Triton-X100 is recommended (e.g. 0.2% in PBS for 5 min). Immunohistochemistry on Paraffin Sections: 1/100. Microwave method is recommended.
Reactivity	Bovine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide (N-terminal amino acids nos. 2-23) of Human Cytokeratin K2 (MW 65,852).
Specificity	Ks 2.342.7.4 represents an excellent marker to study terminal epidermal differentiation. The Monoclonal antibody is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K10). It is also reactive on hyperkeratosis of diverse viral and genetic origin. The Mab does not react with palate keratin K76. Reactive Polypetide: Basic human keratin K2 (MW 65,852; formerly also designated Cytokeratin 2e).
Formulation	State: Supernatant State: Liquid Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 2
Database Link	Entrez Gene 3849 Human UniProt ID P35908
Background	Keratins are the major gene product of keratinocytes and form the intermediate filament cytoskeletal network in these cells. In cells of the upper spinous layer, KRT2E and KRT9 are expressed. Although the expression of KRT9 is limited to palmoplantar epidermis, KRT2E is expressed not only in this tissue but also in other regions, notably the epidermis covering the knee, thigh, and groin. It is not known whether these keratins simply replace their respective type I or type II counterpart in the preexisting KRT1/KRT10 network or dimerize with another, as yet undiscovered keratin partner.
Synonyms	KRT2, KRT2A, KRT2E, Cytokeratin-2e, CK2e, K2e, Keratin-2
Note	*Bovine: Calf hoof epidermis, snooze epithelium; negative with tongue epithelium **Mouse/Rat: Heterogenously on foot sole epidermis only, negative with epidermis of other body sites.
Reference Data

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