POLR2A (794-822) Mouse Monoclonal Antibody [Clone ID: ARNA-3]

Specifications

Product Data
Clone Name	ARNA-3
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting (Western blot). Immunohistochemistry (1/100). Suitable for Cell Culture and Frozen Tissue.  Recommended Fixation conditions for Cells: Incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubation time: 1 h at RT for Immunohistochemistry.
Reactivity	Drosophila, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified RNA polymerase II.
Specificity	ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA. Cultured Cell Lines: Reacts with Drosophila chromosomes, PTK-2, HeLa, Hep2. Epitope Recognized: 794DDYGPESRGFVENSYLA822.
Formulation	State: Supernatant State: Culture Supernatant Concentrate Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	polymerase (RNA) II subunit A
Database Link	Entrez Gene 5430 Human UniProt ID P24928
Background	This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]
Synonyms	RNA polymerase II, RNA polymerase II subunit B1, POLR2, POLRA, RPOL2, RPB1, RPB220
Reference Data
Protein Pathways	Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

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