Transferrin (TF) (N-term) Mouse Monoclonal Antibody [Clone ID: HTF-14]

Specifications

Product Data
Clone Name	HTF-14
Applications	ELISA, FN, IF, IHC, IP, R, WB
Recommend Dilution	ELISA. RIA. Western Blotting (non-reducing conditions). Immunocytochemistry. Functional Application: This antibody blocks binding of Transferrin to the Receptor. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Positive Control Tissue: Placenta.
Reactivity	Human, Porcine, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Porcine Transferrin.
Specificity	The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. Negative Species: Bovine, Sheep, Canine (Dog) and Equine (Horse).
Formulation	Phosphate buffered saline (PBS), pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Precipitation Methods and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	transferrin
Database Link	Entrez Gene 7018 Human UniProt ID P02787
Background	Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 mmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Synonyms	Serotransferrin, Siderophilin
Reference Data

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