Progesterone Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1/50.   Immunohistochemistry on Paraffin Sections: 1/50 Positive Control Tissue: Breast carcinoma and normal young ovarian tissue.  Progesterone antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Reactivity	var.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Progesterone conjugated to BSA
Specificity	This antibody is specific for Progesterone. In normal tissues it stains granulosa cells and theca cells. It can also be used to stain tumourous Sertoli-Leydig, granulosa and theca cells.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Progesteron is a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland. Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta in order to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Progesterone is available as a pharmaceutical that can be used to treat menstrual disorders and for the prevention of miscarriage.
Synonyms	20-dione, pregn-4-ene-3
Reference Data

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