Gastrin (GAST) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1:1,000-1:1,500 Immunohistochemistry on Paraffin Embedded Tissues: 1:1,000-1:1,500 (microwave treatment recommended) Immunofluorescence Microscopy. Recommended Positive Control: Formalin-fixed paraffin sections of Rat antrum. Working Dilutions: 1:1,000-1:1,500 using FITC, with overnight incubation at 2-8°C.
Reactivity	Human, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic Human Gastrin I conjugated to BSA
Specificity	Gastrin-secreting cells are numerous in the antrum and a few are found in the proximal duodenum. The antibody can be used for the diagnosis of Gastrin-producing tumors which are mainly found in the pancreas and occasionally in the stomach and the duodenum. Absorption with 10-100 µg Gastrin 1-34 and CCK 8 per ml antiserum abolishes the staining.
Formulation	State: Serum State: Lyophilized Serum
Reconstitution Method	Restore in 100 µl distilled water
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	gastrin
Database Link	Entrez Gene 2520 Human UniProt ID P01350
Background	Gastrin is mainly known by its first described role as a gastric acid-hormone but the protein also has the ability to promote cellular growth. More recently, gastrin has been suggested to induce leukocyte-endothelial cell interactions and to have a pro-inflammatory effect. Gastrin has two biologically active peptide forms, G34 and G17 and they activate two different receptors: the CCK-1 receptor, which has low affinity for gastrin but high affinity for the related hormone cholecystokinin (CCK), and the CCK-2 receptor, which has high affinity for both gastrin and CCK and mediates the acid-secretory as well as the proliferative effects of gastrin.
Synonyms	GAST, GAS
Reference Data

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