Plectin (PLEC) (C-term) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting: 1/16,000 for Western blot (ECL). Cytological Material. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/500 (enhanced after microwave treatment).  Incubation time: 1 h at RT; extended with paraffin sections.
Reactivity	Bovine, Fish, Human, Mouse, Xenopus
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	C-terminal "C" domain of Human recombinant Plectin (aa 4367-4684).
Specificity	Calculated MW 532,000 protein plectin, localizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes). Human glia cells are specifically detected. Tested Reactivity on Cultured Cell lines: HL-60, Huvec (human umbilical cord endothelial cell line), BMGE, 3T3.
Formulation	State: Serum State: Whole antiserum Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	plectin
Database Link	Entrez Gene 5339 Human UniProt ID Q15149
Background	Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking. Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes. Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions.
Synonyms	PLEC, Plectin-1, PLEC1, PCN, PLTN
Reference Data

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