NPY1R (365-378) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot (1/250 as a guide). Immunohistochemistry on Paraffin Sections (1/1,000-1/4,000): See below for protocol.
Reactivity	Human, Rat
Host	Sheep
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to amino acids 365-378 of human NPY1R (KQASPVAFKKINNN) conjugated to KLH.
Specificity	This antibody reacts with Neuropeptide Y Receptor 1.
Formulation	PBS, pH 7.2, containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Fractionation
Conjugation	Unconjugated
Storage Condition	Can be shipped at 2-8°C. Upon arrival, store the antibody (aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	neuropeptide Y receptor Y1
Database Link	Entrez Gene 4886 Human UniProt ID P25929
Background	Neuropeptide Y (NPY) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. There are two major subtypes of NPY (Y1 and Y2). The NPY Y1 receptors have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, breast, colon, ganglion, heart, and aortic smooth muscle. The Y2 receptor is found mainly in the central nervous system. NPY receptors are a member of the G protein-coupled receptor superfamily.
Synonyms	NPYR, NPYY1, Neuropeptide Y receptor 1
Note	Protocol: Protocol: - Samples: Bouin's fixed paraffin sections, 5 µm, from rat brain. - 5 minutes microwave pre-treatment, citrate buffer, pH 6.0. - Peroxidase blocking (15 minutes), normal rabbit serum (30 minutes), Dako Protein Block (5 minutes). - Primary antibody at 1/2000 (as a guideline). - Overnight incubation at 4ºC. - Secondary antibody: Rabbit anti-sheep IgG. 30 minutes incubation. - 2 hours ABC complex.
Reference Data

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