Col1a1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	ELISA RIA: 1/200 (OD ≥ 500).  Immunofluorescence : ≥ 1/40. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Incubation Time: 60 min at RT or 2-8°C over night. Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Rat skin or liver.
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Collagen type I from Rat skin
Specificity	Rat Collagen type I: 100% Rat Collagen II, III and V: < 0.1% Human, Mouse, Chicken Collagen type I: < 0.1% Rat Elastin: < 0.1% (Determined by RIA at 1/200 dilution).
Formulation	PBS State: Purified State: Lyophilized purified Ig fraction Stabilizer: None Preservative: None
Reconstitution Method	Restore with 0.1 ml distilled water, for further dilution use appropriate antibody diluent.
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	collagen, type I, alpha 1
Database Link	Entrez Gene 29393 Rat UniProt ID P02454
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95kDa) is mainly found in bone, cornea, skin and tendon.
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data

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