Col1a1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	Western Blot. Radioimmunoassay: 1/200. ELISA: ≥ 1/200. Immunfluorescence (Indirect):  ≥ 1/80. Immunohistochemistry on Paraffin Sections:  ≥ 1/500.  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.  Incubation time: 60 min at RT or 2-8°C over night.  Positive Control: Mouse skin.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Collagen type I from Mouse skin
Specificity	This antibody reacts with Mouse Collagen type I (100%), Mouse Collagen type II and IV (< 0.1%), Mouse Collage type III (< 1%), Human, Chicken and Rat Collagen type I (< 0.1%, RIA at 1/200 dilution).
Formulation	Phosphate Buffered Solution without stabilizer or preservatives State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 0.1 ml sterile distilled water. For further dilution use appropriate antibody diluent.
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	collagen, type I, alpha 1
Database Link	Entrez Gene 12842 Mouse UniProt ID P11087
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data

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